gerBC [2019-06-19 13:08:15]

lipoprotein, nutrient receptor, germination response to the combination of glucose, fructose, and KCl

locus: BSU35820

pI: 9.06

mw: 42.31 kDa

protein length: 374 aa

gene length: 1125 bp

function: germination

product: nutrient receptor

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.1|Germinant receptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,691,372 3,692,496

The protein

Protein family

• gerABKC lipoprotein family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|GerAC]

[SW|Domains]

• has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide [Pubmed|23335419]

Structure

• [PDB|3N54] [Pubmed|20654628]

[SW|Localization]

• outer surface of the inner spore membrane [Pubmed|23335419,21685283]

Additional information

• 700 molecules are present per spore [Pubmed|23749970]

Expression and Regulation

Operons

• genes: [gene|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[gene|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]
  description: [Pubmed|8012571]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,8012571], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,8012571]

  additional information

  • 700 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE35820 ([gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACGGAGAATTTTGATGCTG, downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG
• BKK35820 ([gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACGGAGAATTTTGATGCTG, downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG

References

• 23396907,16740944,23749970,24752279,7812448,12670969,15774895,15699190,8012571,16352818,20654628,21685283,21696470,22493018,23335419,26731423